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Web Ecology An open-access peer-reviewed journal
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WE | Articles | Volume 18, issue 2
Web Ecol., 18, 129–141, 2018
https://doi.org/10.5194/we-18-129-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Web Ecol., 18, 129–141, 2018
https://doi.org/10.5194/we-18-129-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Standard article 07 Sep 2018

Standard article | 07 Sep 2018

Production of synthetic tetraploids as a tool for polyploid research

Mariana Castro et al.
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Faye Wedrowicz, Jennifer Mosse, Wendy Wright, and Fiona E. Hogan
Web Ecol., 17, 9–18, https://doi.org/10.5194/we-17-9-2017,https://doi.org/10.5194/we-17-9-2017, 2017
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Cited articles  
Allum, J. F., Bringloe, D. H., and Roberts, A. V.: Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time, Plant Cell Rep., 26, 1977–1984, 2007. 
Ascough, G. D., Van Staden, J., and Erwin, J. E.: Effectiveness of colchicine and oryzalin at inducing polyploidy in Watsonia lepida NE Brown, HortScience, 43, 2248–2251, 2008. 
Barker, M. S., Arrigo, N., Baniaga, A. E., Li, Z., and Levin, D. A.: On the relative abundance of autopolyploids and allopolyploids, New Phytol., 210, 391–398, 2015. 
Bates, D., Maechler, M., Bolker, B., and Walker, S.: lme4: linear mixed-effects models using Eigen and S4, available at: http://CRAN.R-project.org/package=lme4 (last access: January 2018), 2014. 
Bretagnolle, F. and Lumaret, R.: Bilateral polyploidization in Dactylis glomerata L. subsp. lusitanica: occurrence, morphological and genetic characteristics of first polyploids, Euphytica, 84, 197–207, 1995. 
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Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate consequences of polyploidy. We developed a protocol based on seedling exposure to colchicine to obtain neotetraploids from wild diploid plants of Jasione maritima, with potential for being used in other species. High rates of tetraploid conversion were obtained, but survival decreased with increasing colchicine concentration. Therefore, low concentrations were ideal for obtaining neotetraploids.
Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate...
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