Articles | Volume 18, issue 2
https://doi.org/10.5194/we-18-129-2018
https://doi.org/10.5194/we-18-129-2018
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07 Sep 2018
Standard article | Highlight paper |  | 07 Sep 2018

Production of synthetic tetraploids as a tool for polyploid research

Mariana Castro, Sílvia Castro, and João Loureiro

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Production of synthetic tetraploids as a tool for polyploid research M. Castro, S. Castro, and J. Loureiro https://doi.org/10.5281/zenodo.1340550

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Short summary
Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate consequences of polyploidy. We developed a protocol based on seedling exposure to colchicine to obtain neotetraploids from wild diploid plants of Jasione maritima, with potential for being used in other species. High rates of tetraploid conversion were obtained, but survival decreased with increasing colchicine concentration. Therefore, low concentrations were ideal for obtaining neotetraploids.