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Web Ecology An open-access peer-reviewed journal
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WE | Articles | Volume 18, issue 2
Web Ecol., 18, 129–141, 2018
https://doi.org/10.5194/we-18-129-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Web Ecol., 18, 129–141, 2018
https://doi.org/10.5194/we-18-129-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Standard article 07 Sep 2018

Standard article | 07 Sep 2018

Production of synthetic tetraploids as a tool for polyploid research

Mariana Castro et al.
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Production of synthetic tetraploids as a tool for polyploid research M. Castro, S. Castro, and J. Loureiro https://doi.org/10.5281/zenodo.1340550

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Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate consequences of polyploidy. We developed a protocol based on seedling exposure to colchicine to obtain neotetraploids from wild diploid plants of Jasione maritima, with potential for being used in other species. High rates of tetraploid conversion were obtained, but survival decreased with increasing colchicine concentration. Therefore, low concentrations were ideal for obtaining neotetraploids.
Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate...
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